Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 75, Issue 2, Pages 351-361Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkz458
Keywords
-
Funding
- EvoTAR
- large-scale integrated project - European Commission Seventh Framework Program
- University of Copenhagen Research Center for Control of Antibiotic Resistance (UC-Care)
- Da Volterra (Paris, France)
Ask authors/readers for more resources
Background Aminopenicillins with or without a beta-lactamase inhibitor are widely used in both human and veterinary medicine. However, little is known about their differential impact on the gut microbiota and development of antimicrobial resistance. Objectives To investigate changes in the faecal microbiota of dogs treated with amoxicillin or amoxicillin/clavulanic acid. Methods Faeces collected from 42 dogs (21 per treatment group) immediately before, during and 1week after termination of oral treatment with amoxicillin or amoxicillin/clavulanic acid were analysed by culture and 16S rRNA gene sequence analysis. Results In both groups, bacterial counts on ampicillin selective agar revealed an increase in the proportion of ampicillin-resistant Escherichia coli during treatment, and an increased occurrence and proportion of ampicillin-resistant enterococci during and after treatment. 16S rRNA gene analysis showed reductions in microbial richness and diversity during treatment followed by a return to pre-treatment conditions approximately 1week after cessation of amoxicillin or amoxicillin/clavulanic acid treatment. While no significant differences were observed between the effects of amoxicillin and amoxicillin/clavulanic acid on microbial richness and diversity, treatment with amoxicillin/clavulanic acid reduced the abundance of taxa that are considered part of the beneficial microbiota (such as Roseburia, Dialister and Lachnospiraceae) and enriched Escherichia, although the latter result was not corroborated by phenotypic counts. Conclusions Our results suggest a limited effect of clavulanic acid on selection of antimicrobial resistance and microbial richness when administered orally in combination with amoxicillin. However, combination with this beta-lactamase inhibitor appears to broaden the spectrum of amoxicillin, with potential negative consequences on gut health.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available