4.7 Article

Physicochemical Changes and in Vitro Gastric Digestion of Modified Soybean Protein Induced by Lipoxygenase Catalyzed Linoleic Acid Oxidation

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 50, Pages 13978-13985

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b05843

Keywords

soy protein isolate; oxidation; in vitro digestion; FRAP; pepsin diffusivity

Funding

  1. National Natural Science Foundation of China [31671870]
  2. Pearl River S&T Nova Program of Guangzhou [201610010105]
  3. Science and Technology Program of Guangzhou [201807010102]
  4. Special Support Project of Guangdong Province for Science and Technology Innovative Young Talents [2014TQ01N538]
  5. 111 Project [B17018]

Ask authors/readers for more resources

Protein oxidation results in structural modification which affects its digestion. The objective of this work was to investigate the influence of lipoxygenases (LOX) catalyzed linoleic acid (LA) oxidation on the structure and in vitro gastric digests of soybean protein isolate (SPI). Fluorescence recovery after photobleaching (FRAP) was used to evaluate the relationship between pepsin diffusion and gastric digestion. Results indicated that oxidation induced carbonyl formation and loss of free sulfhydryl. Increased surface hydrophobicity and zeta-potential verified the protein unfolding and thus resulted in a small particle size and low fluorescence intensity. Fourier transform infrared spectroscopy (FTIR) showed that oxidation caused the increases in beta-sheets mostly at the expense of alpha-helix and random coils. Fluorescein isothiocyanate (FITC)-pepsin in SPI solution modified with 3 mL LA showed a faster diffusion rate with 80.51 mu m(2)/s as well as a higher DH value of 9.11%, showing that pepsin diffusivity might play an important role in protein gastric digestion.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available