Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 67, Issue 42, Pages 11665-11674Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.9b04731
Keywords
stachyose; inflammation; butanoic acid; gut microbiota
Funding
- National Natural Science Foundation of China [C31671823, C31871752, C31901702]
- Science and Technology Research and Development Program of Shaanxi Province [2019NY-125]
- China Postdoctoral Science Foundation [2019M653535]
- Fundamental Research Founds for the Central Universities of Shaanxi Normal University, Xian, China [GK201801009, GK201803701]
- Development Program for Innovative Research Team of Shaanxi Normal University, China [GK201801002]
- Sci-Tech Innovation Team of Shaanxi Province, China [2019TD-035]
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A long-term high-fat diet (HFD) can cause a range of health problems. Gut microbiota plays a decisive role in the development of HFD-associated inflammation, involved in function of T cells. This study was designed to probe the regulative effects of dietary stachyose, a functional oligosaccharide, on HFD-induced weight gain, inflammation, gut microbiota dysbiosis, and T cell abnormality in C57Bl/6 mice. Mice were divided into three groups which received normal chow, HFD and HFD plus stachyose (400 mg/kg), respectively. Results showed that administration of stachyose diminished the HFD-induced upregulation of serum TNF-alpha level and elevation of peripheral blood leukocyte populations to alleviate the HFD-caused colonic and hepatic inflammation in mice. Analysis of gut microbiota revealed that stachyose improved the intestinal homeostasis of HFD-fed mice by improving the bacterial diversity with the increases in the relative abundances of the Prevotellaceae_NK3B31_group, Parasutterella, Christensenellaceae _R-7_group, and Anaerovorax, as well as the fecal level of butanoic acid, while decreasing the ratio of Firmicutes-to-Bacteroidetes and the abundances of the Lachnospiraceae_N-K4A136_group, Desulfovibrio, Anaerotruncus, Mucispirillum, Roseburia, and Odoribacter. Flow cytometric analysis showed that stachyose antagonized the HFD-induced decrease of peripheral CD4(+) T cell population in mice. Conclusively, these findings suggest that long-term consumption of stachyose can ameliorate the HFD-associated colonic and hepatic inflammation and its complications by modulating gut microbiota.
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