Journal
ISRAEL JOURNAL OF PLANT SCIENCES
Volume 67, Issue 1-2, Pages 16-26Publisher
BRILL
DOI: 10.1163/22238980-20191110
Keywords
Adventitious root; auxin; LED; light quality; Wikstroemia gemmate
Categories
Funding
- Agency for Innovation by Science and Technology in Flanders [IWT 083231]
- Ghent University [BOF 01J11408]
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In vitro propagation of the ornamentally interesting species Wikstroemia gemmata is limited by the recalcitrance to form adventitious roots. In this article, two strategies to improve the rooting capacity of in vitro microcuttings are presented. Firstly, the effect of exogenous auxin was evaluated in both light and dark cultivated stem segments and also the sucrose-content of the medium was varied in order to determine better rooting conditions. Secondly, different spectral lights were evaluated and the effect on shoot growth and root induction demonstrated that the exact spectral composition of light is important for successful in vitro growth and development of Wikstroemia gemmata. We show that exogenous auxin cannot compensate for the poor rooting under unfavorable light conditions. Adapting the culture conditions is therefore paramount for successful industrial propagation of Wikstroemia gemmata.
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