Arenimonas terrae sp. nov., isolated from orchard soil

A Gram-stain-negative, strictly aerobic bacterial strain, designated R29(T), was isolated from orchard soil in Hwasun, South Korea. Cells were non-motile rods with catalase and oxidase activities. Growth was observed at 15-40 degrees C (optimum, 30 degrees C) and pH 7.0-9.0. Ubiquinone-8 was identified as the predominant isoprenoid quinone. Major polar lipids consisted of phosphatidylethanolamine, six phospholipids, an unidentified aminolipid and two unidentified polar lipids. Strain R29(T) contained iso-C-15:0, iso-C 16:0 and summed feature 9 (comprising iso-C-17:1 omega 9c/10-methyl-C-16:0) as major cellular fatty acids. The G+C content of the genomic DNA calculated from the whole-genome sequence was 69.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that R29(T) was most closely related to Arenimonas daejeonensis T7-07(T) and Arenimonas malthae CC-JY-1(T) with sequence similarities of 98.0 and 96.9%, respectively The average nucleotide identity and in silico DNA-DNA hybridization values between R29(T) and the type strains of A. daejeonensis and A. malthae were 87.0 % and 31.6% and 83.4% and 26.1%, respectively. On the basis of the phenotypic, chemotaxonomic and molecular features, strain R29(T) represents a novel species of the genus Arenimonas, for which the name Arenimonas terrae sp. nov. is proposed. The type strain is R29(T) (=KACC 19896(T)=JCM 33216(T)).