Hydrogenophaga borbori sp. nov., isolated from activated sludge

A novel Gram-stain-negative, yellowish-pigmented bacterial strain, designated LA-38(T), was isolated from activated sludge of wastewater treatment plants in Hanam city, South Korea. Cell of LA-38(T) were rod-shaped, aerobic, motile and non-spore-forming. In phylogenetic analyses based on 16S rRNA genes, LA-38(T) clustered with species of the genus Hydrogenophaga and appeared closely related to Hydrogenophaga intermedia DSM 5680(T) (99.2 % similarity), Hydrogenophaga palleronii DSM 63(T) (98.2%), Hydrogrenophaga laconesensis KCTC 42478(T) (98.1%), Hydrogenophaga. atypica DSM 15342(T) (98.1%), Hydrogenophaga defluvii DSM 15341(T) (98.0 %) and Hydrogenophaga taeniospiralis DSM 2082(T) (97.2%). The average nucleotide identities between LA-38T and the closely related strains were 79.3-88.5 %, indicating that LA-38(T) represents a novel species of the genus Hydrogenophaga. The DNA G+C content of the genomic DNA was 69.9 mol% and ubiquinone Q-8 was the predominant respiratory quinone. The major cellular fatty acids (>5 %) were C-16:0, cyclo-C-19:0, C-16:1 omega 7c and/or C(16:1 omega)6c (summed feature 3), and C-18:1 omega 7c and/or C (18:1)omega 6c (summed feature 8). The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, the major polyamines were 2-hydroxyputrescine and putrescine. ANI calculation, physiological and biochemical characteristics indicated that LA-38(T) represents a novel species of the genus Hydrogenophaga, for which the name Hydrogenophaga borbori sp. nov. is proposed. The type strain is LA-38(T) (=KACC 19730(T)=LMG 30805(T)).