Flavobacterium microcysteis sp. nov., isolated from a culture of Microcystis aeruginosa

A Gram-stain-negative, yellow-pigmented, strictly aerobic bacterium, designated strain MaA-Y11(T), was isolated from a culture of Microcystis aeruginosa in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain MaA-Y11(T) was observed at 25-37 degrees C (optimum, 30 degrees C) and pH 6.0-9.0 (optimum, 7.0) and in the presence of 0-1.0% (w/v) NaCl (optimum, 0%). Strain MaA-Y11(T) did not produce flexirubin-type pigments. Strain MaA,-Y11(T) contained iso- C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-1(6:1)omega 7c and/or C-16:1 omega 6c) as major cellular fatty acids and menaquinone-6 as the sole isoprenoid quinone. Phosphatidylethanolamine. three unidentified aminolipids and four unidentified lipids were detected as the major polar lipids. The G+C content of the genomic DNA was 37.0 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain MaA-Y11(T) formed a phyletic lineage with Flavobacterium lindanitolerans IP-10(T) within the genus Flavobacterium. Strain MaA-Y11(T) was most closely related to Flavobacterium lindanitoterans IP-10(T), with a 98.85% 16S rRNA gene sequence similarity, and shared less than 93.87% sequence similarities with other type strains. Average nucleotide identity and in silico DNA-DNA hybridization values between strain MaA-Y11(T) and the type strain of F lindanitolerans were 87.0 and 32.3%, respectively. Here, we conclude that strain MaA,-Y11(T) represents a new species of the genus Flavobacterium, for which the name Flavobacterium microcysteis sp. nov. is proposed. The type strain is MaA-Y11(T) (=KACC 21225 7 =JCM 33501(T)).