Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 20, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/ijms20215425
Keywords
miiuy croaker (Miichthys; miiuy); swim bladder; FPYLRH; antioxidant activity; cytoprotective effect
Funding
- National Natural Science Foundation of China (NSFC) [31872547]
- Public Service Technology Application Research Project of Science and Technology Department of Zhejiang Province [LGN18D060002]
- International S&T Cooperation Program of China [2012DFA30600]
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In our previous research, ten antioxidant pentapeptides including FYKWP, FTGMD, GFEPY, YLPYA, FPPYERRQ, GFYAA, FSGLR, FPYLRH, VPDDD, and GIEWA were identified from the hydrolysate of miiuy croaker (Miichthysmiiuy) swim bladder. In this work, their protective function on H2O2-induced oxidative damage to human umbilical vein endothelial cells (HUVECs) was studied. Results indicated that there was no significant difference in the HUVEC viability between the normal group and the treated groups with the 10 pentapeptides at the concentration of 100 mu M for 24 h (p < 0.05). Furthermore, FPYLRH of 100 mu g/mL extremely significantly (p < 0.001) increased the viability (80.58% +/- 5.01%) of HUVECs with H2O2-induced oxidative damage compared with that of the model group. The protective mechanism indicated that FPYLRH could extremely significantly (p < 0.001) increase the levels of superoxide dismutase (SOD) (211.36 +/- 8.29 U/mg prot) and GSH-Px (53.06 +/- 2.34 U/mg prot) and decrease the contents of reactive oxygen species (ROS) (139.1 +/- 11.8% of control), malondialdehyde (MDA) (13.66 +/- 0.71 nM/mg), and nitric oxide (NO) (4.36 +/- 0.32 mu M/L) at the concentration of 100 mu M in HUVECs with H2O2-induced oxidative damage compared with those of the model group. In addition, FPYLRH dose-dependently protected DNA in oxidative damage HUVECs model. These results suggested that FPYLRH could significantly attenuate the H2O2-induced stress injury in HUVECs and might be used as a potential natural antioxidant in the functional food industries.
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