Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
Volume 101, Issue 13, Pages 1922-1934Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03067319.2019.1691183
Keywords
Fluorescence; simultaneous detection; aptamer; Lead (II); Mercury (II)
Categories
Funding
- National Science Foundation of China [21465010]
- Open Foundation of Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province [PKLHB1918, PKLHB1730]
- Scientific and Technological Research Program of the Chongqing Municipal Education Commission [KJ1401021]
- Hubei Provincial Natural Science Foundation of China [2018CFB247]
- Special Funds for Double First-Class Construction in Hubei Province
Ask authors/readers for more resources
A fluorescence quantitative analysis method based on nucleic acid aptamer molecular beacon (MB) probes has been developed for simultaneous detection of Hg2+ and Pb2+. The method shows high sensitivity, accuracy, and a good linear relationship between fluorescence intensity and target concentrations.
We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg2+ and Pb2+ based on nucleic acid aptamer molecular beacon (MB) probes. In this analytical method, two MB probes for Hg2+ (P-Hg) and Pb2+ (P-Pb) were designed. The carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were selected as fluorophores of P-Hg and P-Pb, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were selected as organic quenchers, and several continuous nucleotides with guanine (G base) were connected to organic quenchers. The aptamers are put in as a part of stem and loop. In general, the fluorescence of fluorophores was dually quenched by BHQ and G bases, so the fluorescence signals were weak. In the presence of Hg2+ and Pb2+, P-Hg and P-Pb bonded with them, and the stem-loop structure of MB was destroyed and the fluorescence recovered. Under the optimal conditions, the fluorescence intensity of FAM had a good linear relationship with the concentration of Hg2+ in the range from 0.7 nmol/L to 84 nmol/L, and that of TAMRA and Pb2+ in the range from 0.2 nmol/L to 24 nmol/L. The detection limit of Hg2+ is 0.36 nmol/L and that of Pb2+ is 0.16 nmol/L (3 sigma, n = 11). The relative standard deviations (RSD) for determination of Hg2+ and Pb2+ were both lower than 5%, the average recoveries of this method in real samples were 96.55-102.78%, which indicated that the method had a high accuracy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available