Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 140, Issue -, Pages 568-576Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.08.149
Keywords
A polysaccharide; Okra; Diabetic nephropathy
Funding
- National Natural Science Foundation of China [81573580]
- Key Laboratory of Polysaccharide Bioactivity Evaluation of TCM of Liaoning Province
- Key Techniques Study of Consistency Evaluation of Drug Quality and Therapeutic Effect [18-400-4-08]
- Liaoning Distinguished Professor Project
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Diabetic nephropathy (DN) with high morbidity and mortality is one of the most severe diabetes complications and affects nearly one-third of people with diabetes. Our present experiment was designed to assess the potential therapeutic of a polysaccharide purified from okra (OP) on DN in high-fat diet-fed and streptozotocin (STZ)-induced diabetic mice. We found that an 8-week treatment with OP could significantly decrease the 24-h urine protein (24-h UP), serum creatinine (Scr), serum urea nitrogen (SUN) and glycosylated hemoglobin (HbA1c) levels, which are regard as the biomarkers of renal injury. The results of immunohistochemical analysis and histopathological examination showed that the diabetic-induced microstructural changes and fibrosis in kidney can be alleviated by the administration of OP (400 mg/kg). Our immunofluorescences results demonstrated that OP (400 mg/kg) could greatly reduce the level of reactive oxygen species (ROS) in kidney. In addition, we also studied the level of SOD, GSH, CAT, HO-1, Nrf2, p-AMPK, PGC-1 alpha, Sirt1, Bcl-2, cleaved caspase-3 and Bax in renal tis- sue by assay kit and western blot. Our results suggested that OP ameliorated DN in diabetic mice, which is possibly related to suppressing apoptosis and oxidative stress through activating AMPK-Sirt1-PGC-1 alpha signaling axis. (C) 2019 Elsevier B.V. All rights reserved.
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