Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 153, Issue -, Pages 1281-1290Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.10.263
Keywords
Lentinula edodes; Soluble dietary fiber; Hypolipidemic; Interaction mechanism; Pancreatic lipase; ITC
Funding
- Tianjin Municipal Science and Technology Foundation [18PTZWHZ00190]
- National Natural Science Foundation of China [31371879]
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Aimed to evaluate the hypolipidemic effects of high molecular weight soluble dietary fiber extracted from L edodes (LEHSDF), this study investigated the structure and interaction mechanism of LEHSDF with pancreatic lipase (EL) and bile salts (BS) that were involved in lipid digestion. 1D/2D NMR spectra indicated that the main chain of LEHSDF consisted of (1 -> 2,4)-linked beta-D-arabinopyranosyl, (1 -> 3)-linked alpha-L-rhamnopyranosyl, (1 -> 4)-linked beta-D-xylopyranosyl, (1 -> 6)-linked and (1 -> 4)-linked beta-D-glucopyranosyl, with beta-D-galactopyranosyl and alpha-D-mannopyranosyl as terminal unit. Oil red O staining results suggested that LEHSDF had an effective inhibitory effect on lipid accumulation in HepG2 cells. Isothermal titration calorimetry, fluorescence and circular dichroism spectra showed that BS did not specifically bind to LEHSDF, and the strong inhibitory effect of LEHSDF on lipase was dominated by hydrophobic forces, electrostatic forces, encapsulation and adsorption interactions. The results will be helpful for the design of food containing LEHSDF as a functional additive to control lipid digestion. (C) 2019 Elsevier B.V. All rights reserved.
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