Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 153, Issue -, Pages 1176-1185Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.10.247
Keywords
Exopolysaccharides; Glutamicibacter halophytocola; Antioxidant activity
Funding
- National Natural Science Foundation of China [31370062]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [17KJA180004, 18KJB180006]
- Promoting Science and Technology Innovation Project of Xuzhou City [KC18142]
- Qing Lan Project of Jiangsu Province
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In this study, an endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180, was investigated for the production and antioxidant activity of exopolysaccharides (EPSs). First, the suitable fermentation time, temperature, inoculation volume, pH value, and the carbon and nitrogen sources for EPSs production were obtained using the one variable at a time method (OVAT). Then, a central composition design was used for fermentation conditions optimization to obtain the maximum EPS yield. The optimal medium and condition were as follows: 100 mL broth in 250 mL Erlenmeyer flasks, including 3.65 g/L maltose, 9.88 g/L malt extract, 3.40 g/L yeast extract, 1.41 g/L MnCl2, pH 7.5, culture temperature 28 degrees C, and 200 rpm for 7 days, which increased the yield of EPSs to 2.89 g/L. Two purified EPSs, 5180EPS-1 (MW 58.9 kDa) and 5180EPS-2 (10.5 kDa), comprising rhamnose, galacturonic acid, glucose, glucuronic acid, xylose, and arabinose, were obtained for chemical analysis and antioxidant evaluation. The scavenging ability and reducing power of the superoxide anion and hydroxyl radicals demonstrated the moderate in vitro antioxidant activities of the two EPSs, thus indicating their potential to be a new source of natural antioxidants. However, further structure elucidation and functional studies need to be continued. (C) 2019 Elsevier B.V. All rights reserved.
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