Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 140, Issue -, Pages 907-919Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.08.161
Keywords
Dandelion root polysaccharides; Response surface methodology; Antioxidant activity
Funding
- Doctoral Scientific Research Foundation of Affiliated Hospital of Nantong University [Tdb19014]
- Science and Technology Program of Nantong [JC218128]
- Scientific Research Foundation of Nantong Pharmaceutical Association [ntyx1806]
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In this study, response surface methodology (RSM) was used to optimize the extraction parameters of dandelion root polysaccharide (DRP). Two novel polysaccharides (DRP-2b, DRP-3a) were isolated from dandelion root by graded ethanol precipitation and column chromatography. Structural analyses indicated that DRP-2b, with a molecular weight of 31.8 kDa, was composed of rhamnose, glucuronic acid, glucose, galactose and arabinose, whereas DRP-3a, with a molecular weight of 6.72 kDa, was composed of rhamnose, glucose, galactose and arabinose. The backbones of DRP-2b and DRP-3a were mainly composed of (1 -> 5)-linked-alpha-D-Ara and (1 -> 6)-linked-alpha-D-Glc, respectively. In addition, the antioxidant activities and protective effects against H2O2-induced damage in hepatic L02 cells were evaluated. DRP-3a exhibited higher radical scavenging activity than DRP-2b against the DPPH, hydroxyl and superoxide anions. Furthermore, DRP-3a exhibited strong protective effects against H2O2-induced damage in hepatic L02 cells. Thus, DRP-3a could be used as a potential antioxidant in medicine or as a functional food. (C) 2019 Published by Elsevier B.V.
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