Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 139, Issue -, Pages 30-39Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.07.090
Keywords
Zein; Chondroitin sulfate; Antisolvent precipitation
Funding
- National Natural Science Foundation of China [31571927]
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The purpose of this work is to stabilize zein nanoparticles with anionic polysaccharides-chondroitin sulfate (CS) to overcome the poor colloidal stability of zein nanoparticles. The average molecular weight of CS was 20.51 kDa and the disaccharide composition of CS was 70.71% monosulfated disaccharide Di2S and 29.29% disulfated disaccharide Di2,6 diS. Zein/CS composite nanoparticles were fabricated by antisolvent precipitation method at pH4.0. The optimal mass ratio of zein to CS was 1:1 (w/w). Zein/CS composite nanoparticles showed mean size of 148 nm and PDI < 0.2, maintaining great stability at pH 3.0 to 8.0. Meanwhile, these prepared nanoparticles were also stable to heat treatment and was stable to a range of ionic strength of 0-15 mM at pH 4.0 and 0-10 mM at pH 7.0. The composite nanoparticles exhibited regular spherical structures, and CS was deposited on the surface of zein mainly by electrostatic interactions. In the process, the addition of urea and sodium dodecyl sulfate indicated that it also involved both hydrogen bonding and hydrophobic interactions. In addition, cytotoxicity assay on human renal epithelial cells-293 cells indicated that zein/CS composite nanoparticles were essentially nontoxic. (C) 2019 Elsevier B.V. All rights reserved.
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