Journal
INNATE IMMUNITY
Volume 26, Issue 4, Pages 294-300Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425919886643
Keywords
Leptin; NLRP3 inflammasome; systemic lupus erythematosus; Th17 cells; MRL; Mp-Fas lpr mice
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Funding
- Science and Technology Innovation Special Fund for Medical Health Project of BaoShan District [18-E-25]
- National Natural Science Foundation of China [81401345, 81601396, 31670885]
- Young Physician Training Plan of North Huashan Hospital, Fudan University [0000077]
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Both NLRP3 inflammasome and Th17 cells play important roles in the pathogenesis of systemic lupus erythematosus (SLE). Here we tried to investigate whether leptin promotes the differentiation of Th17 cells from lupus mice by activating the NLRP3 inflammasome. Th17 cells induced from MRL/Mp-Fas lpr mice splenocytes under Th17 polarizing condition were treated with leptin at scalar doses during the last 18 h of culture. The mRNA levels of IL-17A, IL-17F, ROR gamma t, IL-1 beta, IL-18, NLRP3, ASC, and IL-1R1 were detected by quantitative PCR. IL-17A, IL-17F, IL-1 beta, and IL-18 were tested by ELISA, while the activity of caspase-1 and number of Th17 cells were counted by flow cytometry before/after inhibition of the NLRP3 inflammasome. We found that leptin pushed up the expression of IL-17A, IL-17F, NLRP3, and IL-1 beta and increased the number of Th17 cells in lupus mice, while the expression of IL-17A, ROR gamma t, and IL-1 beta and the number of Th17 cells were decreased after inhibition of the NLRP3 inflammasome. Leptin promoted the differentiation of Th17 cells from lupus mice by activating the NLRP3 inflammasome.
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