Journal
INFECTION GENETICS AND EVOLUTION
Volume 75, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.meegid.2019.103945
Keywords
Feline kidney cell line; microRNA; FPV; Deep sequencing
Categories
Funding
- Agricultural Science and Technology Innovation Program [ASTIP-IAS15]
- National Natural Science Foundation of China [31172349, 31172341]
- National Key Research and Development Program of China [2016YFD0501003, 2017YFD0502300]
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MicroRNAs (miRNAs) play crucial roles in post-transcriptional regulation of gene expression in many biological processes. Feline panleukopenia virus (FPV) is a highly infectious pathogen that can cause severe disease in pets, economically important animals and wildlife. In this study, miRNAs associated with FPV infection were identified using high-throughput sequencing. Our results showed that 673 known miRNAs and 278 novel miRNAs were identified and 57 significantly differential expression miRNAs were found post-FPV infection in feline kidney cell line. Stem-loop qRT-PCR was applied to validate the expression of the randomly selected miRNAs; the results were consistent with the sequencing data. Furthermore, the target genes of differential expression miRNAs were analyzed and predicated by GO and KEGG pathway. Altogether, our analysis provides a potential link between miRNA expression and the pathogenesis of FPV infection.
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