Journal
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
Volume 58, Issue 47, Pages 21201-21207Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.iecr.9b03622
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Funding
- National Natural Science Foundation of China [51802273]
- Science and Technology Department of Sichuan Province project [19YYJC2882]
- Foundation of Sichuan Educational Committee [14ZB0050]
- Young Scholars Development Fund of SWPU [201331010080]
- School Technology Fund of Southwest Petroleum University [2013XJZ016]
- College Students' Innovative Entrepreneurial Training Projects in Sichuan Province [201810615090]
- Key Project of Southwest Petroleum University Open Experiments [KSZ18427]
- Ordinary Project of Southwest Petroleum University Open Experiments [KSP18414]
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Intake of water contaminated with mercury ions leads to various toxic effects and health issues. In this work, a novel gold nanoparticles (AuNPs) DNA hybrid sensor for fluorescent detection of mercury(II) ions (He2+) in aqueous solution was prepared. The fluorescein was modified onto the terminal of ssDNA and it consequently presented a strong fluorescence. The probing behaviors toward various metal ions were investigated through the fluorescence intensity change. The result demonstrated that the amplification strategy based on ssDNA-AuNPs for Hg2+ detection by using DNase I exhibited high sensitivity and selectivity. Under optimum condition, the resultant chemosensor tested the concentration of mercury ions in a linear range from 10 to 300 nM (R-2 = 0.9637), and a detection limit as low as 2.11 nM. Moreover, the developed sensor was used to detect Hg2+ ions in the real samples, and the observed results were in good coordination with the value of calculation.
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