Journal
BIOMATERIALS
Volume 77, Issue -, Pages 255-266Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.10.026
Keywords
Mesenchymal stem cells (MSCs); Human umbilical vein endothelial cells (HUVECs); Poly(propylene fumarate) (PPF); Fibrinogen; Thrombin
Funding
- NIH [R01-AR061460, P30 CA016058]
- Veterans Administration
- NATIONAL CANCER INSTITUTE [P30CA016058] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR061460] Funding Source: NIH RePORTER
- Veterans Affairs [I01BX000418] Funding Source: NIH RePORTER
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The importance of vascularization in the field of bone tissue engineering has been established by previous studies. The present work proposes a novel poly(propylene fumarate) (PPF)/fibrin composite scaffold for the development of vascularized neobone tissue. The effect of prevascularization (i.e., in vitro pre-culture prior to implantation) with human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on in vivo vascularization of scaffolds was determined. Five conditions were studied: no pre-culture (NP), 1 week pre-culture (1P), 2 week pre-culture (2P), 3 week pre-culture (3P), and scaffolds without cells (control, C). Scaffolds were implanted subcutaneously in a severe combined immunodeficiency (SCID) mouse model for 9 days. During in vitro studies, CD31 staining showed a significant increase in vascular network area over 3 weeks of culture. Vascular density was significantly higher in vivo when comparing the NP and 3P groups. Immunohistochemical staining of human CD-31 expression indicated spreading of vascular networks with increasing pre-culture time. These vascular networks were perfused with mouse blood indicated by perfused lectin staining in human CD-31 positive vessels. Our results demonstrate that in vitro prevascularization supports in vivo vascularization in PPF/fibrin scaffolds. (C) 2015 Elsevier Ltd. All rights reserved.
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