Journal
BIOMATERIALS
Volume 77, Issue -, Pages 280-290Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2015.10.045
Keywords
Vascularization; Immunomodulation; Angiogenesis; Arteriogenesis; SDF-1; Hydrogel
Funding
- National Institutes of Health [K01AR052352-01A1, R01AR056445-01A2, R01DE019935-01]
- Petit Faculty Fellowship funds
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [K01AR052352, R01AR056445] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [R01DE019935] Funding Source: NIH RePORTER
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Tissue repair processes are characterized by the biphasic recruitment of distinct subpopulations of blood monocytes, including classical (inflammatory) monocytes (IMs, Ly6C(hi)Gr1(+)CX3CR1(lo)) and non-classical anti-inflammatory monocytes (AMs, Ly6C(lo)Gr1(-)CX3CR1(hi)). Drug-eluting biomaterial implants can be used to tune the endogenous repair process by the preferential recruitment of pro-regenerative cells. To enhance recruitment of AMs during inflammatory injury, a novel N-desulfated heparin-containing poly(ethylene glycol) diacrylate (PEG-DA) hydrogel was engineered to deliver exogenous stromal derived factor-1 alpha (SDF-1 alpha), utilizing the natural capacity of heparin to sequester and release growth factors. SDF-1 alpha released from the hydrogels maintained its bioactivity and stimulated chemotaxis of bone marrow cells in vitro. Intravital microscopy and flow cytometry demonstrated that SDF-1 alpha hydrogels implanted in a murine dorsal skinfold window chamber promoted spatially-localized recruitment of AMs relative to unloaded internal control hydrogels. SDF-1 alpha delivery stimulated arteriolar remodeling that was correlated with AM enrichment in the injury niche. SDF-1 alpha, but not unloaded control hydrogels, supported sustained arteriogenesis and microvascular network growth through 7 days. The recruitment of AMs correlated with parameters of vascular remodeling suggesting that tuning the innate immune response by biomaterial SDF-1 alpha release is a promising strategy for promoting vascular remodeling in a spatially controlled manner. (C) 2015 Elsevier Ltd. All rights reserved.
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