Journal
BIOMATERIALS
Volume 87, Issue -, Pages 1-17Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.02.012
Keywords
Polydopmine; Carboxymethyl chitosan; Peptide; Human embryonic stem cells; Human induced pluripotent stem cells; Chemically-defined
Funding
- National Natural Science Foundation of China [81371697]
- Open Project of Key Laboratory of Regenerative Biology [KLRB201401]
- Peking University's 985 Grant
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Human pluripotent stem cells (hPSCs) possess great value in the aspect of cellular therapies due to its self-renewal and potential to differentiate into all somatic cell types. A few defined synthetic surfaces such as polymers and adhesive biological materials conjugated substrata were established for the self renewal of hPSCs. However, none of them was effective in the generation of human induced pluripotent stem cells (hiPSCs) and long-term maintenance of multiple hPSCs, and most of them required complicated manufacturing processes. Polydopamine has good biocompatibility, is able to form a stable film on nearly all solid substrates surface, and can immobilize adhesive biomolecules. In this manuscript, a polydopamine-mediated surface was developed, which not only supported the reprogramming of human somatic cells into hiPSCs under defined conditions, but also sustained the growth of hiPSCs on diverse substrates. Moreover, the proliferation and pluripotency of hPSCs cultured on the surface were comparable to Matrigel for more than 20 passages. Besides, hPSCs were able to differentiate to cardiomyocytes and neural cells on the surface. This polydopamine-based synthetic surface represents a chemically-defined surface extensively applicable both for fundamental research and cell therapies of hPSCs. (C) 2016 Elsevier Ltd. All rights reserved.
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