Journal
BIOMATERIALS
Volume 85, Issue -, Pages 78-87Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.01.064
Keywords
Surface functionalization; Polystyrene nanoparticles; Macrophage subsets; Immunomodulation; Cytokine
Funding
- Deutsche Forschungsgemeinschaft (DFG) through the Priority Program [SPP1313 (Si 285/5-2)]
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Macrophages are key regulators of innate and adaptive immune responses. Exposure to microenvironmental stimuli determines their polarization into proinflammatory M1 and anti-inflammatory M2 macrophages. M1 exhibit high expression of proinflammatory TNF-alpha and IL-1 beta, and M2 promote tissue repair, but likewise support tumor growth and cause immune suppression by expressing IL-10. Thus, the M1/M2 balance critically determines tissue homeostasis. By using carboxyl- (PS-COOH) and aminofunctionalized (PS-NH2) polystyrene nanoparticles, the effects of surface decoration on the polarization of human macrophages were investigated. The nanoparticles did not compromise macrophage viability nor did they affect the expression of the M1 markers CD86, NOS2, TNF-alpha, and IL-1 beta. By contrast, in M2, both nanoparticles impaired expression of scavenger receptor CD163 and CD200R, and the release of IL-10. PS-NH2 also inhibited phagocytosis of Escherichia coli by both, M1 and M2. PS-COOH did not impair phagocytosis by M2, but increased protein mass in M1 and M2, TGF-beta 1 release by M1, and ATP levels in M2. Thus, nanoparticles skew the M2 macrophage polarization without affecting M1 markers. Given the critical role of the M1 and M2 polarization for the immunological balance in patients with cancer or chronic inflammation, functionalized nanoparticles might serve as tools for reprogramming the M1/M2 polarization. (C) 2016 Elsevier Ltd. All rights reserved.
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