Journal
BIOMATERIALS
Volume 106, Issue -, Pages 78-86Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.08.020
Keywords
Circulating tumor cells; Magnetic nanowire; Conducting polymer; Colorimetric detection; Breast cancer
Funding
- National Cancer Center grant from the Republic of Korea [1611170-1]
- Korea Health Promotion Institute [1611170-1] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Circulating tumor cells (CTCs) are recognized as promising biomarkers for diagnosis and indication of the prognosis of several epithelial cancers. However, at present, CTC monitoring is available only for advanced-stage patients rather than for those at an early stage of cancer. This is because of the extraordinary rarity of CTCs and the limited sensitivity of current methods. Herein, we report the development of multifunctional magnetic nanowires for the efficient isolation and detection of CTCs from the blood of patients, especially those with non-metastatic early-stage cancer. The nanowires, which are equipped with a high density of magnetic nanoparticles and five different types of antibodies (Ab mixture_mPpyNWs), offer a significant improvement in cell-isolation efficiency, even from very small amounts of blood (250 mu L-1 mL). Notably, CTCs were isolated and identified in 29 out of 29 patients (100%) with non-metastatic early breast cancer, indicating that this procedure allowed detection of CFCs with greater accuracy, sensitivity, and specificity. In addition, we demonstrated in situ naked eye identification of the captured cancer cells via a simple colorimetric immunoassay. Our results show that antibody-functionalized magnetic nanowires offer great potential for a broad range of practical clinical applications, including early detection, diagnosis, and treatment of cancer. (C) 2016 Elsevier Ltd. All rights reserved.
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