Journal
BIOMARKERS
Volume 22, Issue 6, Pages 575-583Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/1354750X.2016.1252959
Keywords
Polycyclic aromatic hydrocarbons; carcinogens; diesel exhaust; method validation; tobacco smoke; general population
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Context: Several urinary PAHs metabolites can be detected by HPLC-MS/MS for individual exposure assessment. Objective: Quantitation of urinary metabolites of four PAHs, selected on the basis of their significance, with reduced costs and high sensitivity. Materials and methods: HPLC-MS/MS was used and pure standards and isotope-labeled internal analogs of the analytes. Two hundred samples were tested after enzymatic hydrolysis. Results: Accuracy was higher than 90% and variability lower than 19%; LODs permit to measure 1-hydroxypyrene, 1 and 2-hydroxynaphthalene in all subjects, 6-hydroxynitropyrene in the 65% and 3-hydroxybenzo[a]pyrene in the 70%. Discussion and conclusion: The method is suitable both for occupational and for environmental studies. This is the first paper reporting urinary levels of 6-hydroxynitropyrene in European subjects, nonoccupationally exposed to nitro-PAHs.
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