Journal
BIOMARKERS
Volume 21, Issue 8, Pages 743-751Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/1354750X.2016.1201533
Keywords
Baseline concentration; inflammation biomarker; mass spectrometry; polymorphism; posttranslational modifications; proteoforms
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Funding
- National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R01DK082542, R24DK090958, K23HL107389]
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Objective: Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states. Methods: We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms. Results: Intra-and inter-day precision revealed CVs <10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman-Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples revealed 7 diverse SAA polymorphic types and 12 different proteoforms. Conclusion: The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.
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