Measurement of aflatoxin M1 in powder and pasteurized milk samples by using a label-free electrochemical aptasensor based on platinum nanoparticles loaded on Fe-based metal-organic frameworks

In the present study, a sensitive label-free electrochemical aptasensor is introduced to measure aflatoxin M1 (AFM1) by using platinum nanoparticles (PtNPs) decorated on a glassy carbon electrode (GCE) modified with Fe-based metal-organic frameworks, MIL-101(Fe). The MIL-101(Fe) and the PtNP/MIL-101(Fe) are synthesized and characterized by Fourier transform infrared spectroscopy, X-ray diffraction, UV-Visible spectroscopy, and field-emission scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are done to monitor the fabrication processes of the aptasensor. In optimum conditions, the linear calibration range of 1.0 x 10(-2) to 80.0 ng mL(-1) and the detection limit of 2.0 x 10(-3) ng mL(-1) are obtained to measure AFM1 concentration using the EIS method. Finally, the fabricated aptasensor is successfully applied to measure AFM1 concentration in powder and pasteurized milk samples.