Journal
FOOD CHEMISTRY
Volume 297, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125035
Keywords
Electrochemical detection system; Lactobacillus casei 1580; Branch-chain aminotransferases; Nicotinamide adenine dinucleotide
Funding
- National Key R&D Program of China [2016YFD0401501]
- Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX(18)2003]
- Yangzhou City Science and Technology Project [YZ2016057, YZ2016047]
- State Key Laboratory for meat quality and safety control [YR2018005]
- National Natural Science Foundation of China [31371792]
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In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB). A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor. The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM). Results indicated that the MWCNTs-CS/GCE electrode exhibited higher stability and sensitivity, compared with the GCE electrode. The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 mu M and the response limit was 0.12 mu M. The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method. The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.
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