Journal
FOOD CHEMISTRY
Volume 298, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.125011
Keywords
Certified reference material; Japanese scallop (Mizuhopecten yessoensis); Okadaic acid; Dinophysistoxin-1; Liquid chromatography-tandem mass spectrometry; Standard addition method; Co-laboratory study; Uncertainty
Funding
- Cross-Ministerial Strategic Innovation Promotion Program (Technologies for Creating Next-Generation Agriculture, Forestry and Fisheries) - Bio-Oriented Technology Research Advancement Institution, Japan
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A scallop midgut gland certified reference material, NMIJ CRM 7520-a, was developed for validation and quality assurance during the inspection of shellfish for diarrhetic shellfish toxins. The candidate material was prepared by using naturally-toxic and nontoxic boiled midgut glands spiked with okadaic acid (OA). The homogeneity and stability of the material were found to be appropriate. For the characterization of OA and dinophysistoxin-1 (DTX1), nine participants were involved in a co-laboratory study based on the Japanese Official Testing Method, where the compounds were assayed by liquid chromatography-tandem mass spectrometry following alkaline hydrolysis. The analytical values were obtained by the standard addition method with a standard spiking solution calibrated using the standard-solution certified reference materials OA and DTX1. The certified concentrations with expanded uncertainties (coverage factor k=2, approximate 95% confidence interval) were determined to be (0.205 +/- 0.061) mg/kg for OA and (0.45 +/- 0.11) mg/kg for DTX1.
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