Journal
FOOD CHEMISTRY
Volume 297, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.124965
Keywords
Graphene oxide; Fluorescent lateral flow; E. coli O157:H7; Food safety; Minced beef; Water quality
Funding
- Science and Technology Development Fund (STDF), Egypt [25347]
- CONACYT (Mexico) [293523]
- National System of Researchers, CONACYT (Mexico) [74314]
- Severo Ochoa program from Spanish MINECO [SEV-2017-0706, MAT2017]
- CERCA Programme/Generalitat de Catalunya
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Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157: H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g(-1) or mL(-1), respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water.
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