Journal
FOOD AND CHEMICAL TOXICOLOGY
Volume 135, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fct.2019.110934
Keywords
Bacteroides fragilis; Safety assessment; Next-generation probiotics; Antibiotic resistance; Virulence factors; Immunosuppressed mice
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Funding
- National Natural Science Foundation of China [31871773, 31470161, 31530056]
- Natural Science Foundation of Jiangsu Province [BK20160175]
- National First-Class Discipline Program of Food Science and Technology [JUFSTR20180102]
- Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
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The novel commensal strain of Bacteroides fragilis HCK-B3 isolated from a healthy Chinese donor was discovered beneficial effects of attenuating lipopolysaccharides-induced inflammation. In order to contribute to the development of natural next-generation probiotic strains, the safety assessment was carried out with in vitro investigations of its morphology, potential virulence genes and antimicrobial resistance, and an in vivo acute toxicity study based on both healthy and immunosuppressed mice by cyclophosphamide injection. Consequently, the potential virulence genes in the genome of B. fragilis HCK-B3 have yet been identified as toxicity-associated. The absence of plasmids prevents the possibility of transferring antibiotic resistance features to other intestinal commensals. No intracorporal pathogenic properties were observed according to the body weight, hematological and liver parameters, cytokine secretions and tissue integrity. In addition, B. fragilis HCK-B3 performed alleviations on part of the side effects caused by the cyclophosphamide treatment. Thus, the novel strain of B. fragilis HCK-B3 was confirmed to be non-toxigenic and did not display adverse effects in both healthy and immune-deficient mice at a routinely applicable dose.
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