Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 94, Issue -, Pages 239-248Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2019.09.008
Keywords
Flow cytometry; Molluscan immunology; Innate immunity; Molluscan haemocytes; Immunophenotyping
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Flow cytometry (FCM) is routinely used in fundamental and applied research, clinical practice, and clinical trials. In the last three decades, this technique has also become a routine tool used in immunological studies of molluscs to analyse physical and chemical characteristics of haemocytes. Here, we briefly review the current implementation of FCM in the field of molluscan immunology. These applications cover a diverse range of practices from straightforward total cell counts and cell viability to characterize cell subpopulations, and further extend to analyses of DNA content, phagocytosis, oxidative stress and apoptosis. The challenges and prospects of FCM applications in immunological studies of molluscs are also discussed.
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