Journal
BIOMACROMOLECULES
Volume 17, Issue 12, Pages 3922-3930Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.6b01285
Keywords
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Funding
- NSFC [21174107, 21374080, 21674081, 21611130175]
- Shanghai 1000 Talents Plan
- Shanghai International Scientific Collaboration Fund [15230724500]
- Fundamental Research Funds for the Central Universities [0500219211, 1500219107]
- Beijing National Laboratory for Molecular Sciences [BNLMS20140127]
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Traditional antibiotics usually sterilize in chemical ways, which may lead to serious drug resistance. By contrast, peptide-based antibacterial materials are less susceptible to drug resistance. Herein we report the preparation of an antibacterial peptide-based copolymer micelle and the investigation of its membrane-penetration antibacterial mechanism by transmission electron microscopy (TEM). The copolymer is poly(L-lactide)-block-poly(phenylalanine-stat-lysine) [PLLA(31)-b-poly(Phe(24)-stat-Lys(36))], which is synthesized by ring-opening polymerization. The PLLA chains form the core, whereas the polypeptide chains form the coronas of the micelle in aqueous solution. This micelle boasts excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria. Furthermore, TEM studies clearly reveal that the micelles pierce and then destroy the cell membrane of the bacteria. We also compared the advantages and disadvantages of two general methods for measuring the Minimal Inhibitory Concentration (MIC) values of antibacterial micelles. Overall, this study provides us with direct evidence for the antibacterial mechanism of polypeptide-based micelles and a strategy for synthesizing biodegradable antibacterial nano materials without antibiotic resistance.
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