Journal
FEBS LETTERS
Volume 594, Issue 4, Pages 611-624Publisher
WILEY
DOI: 10.1002/1873-3468.13631
Keywords
14-3-3 proteins; ferroptosis; glutathione peroxidase 4; protein; protein interaction; redox regulation; RSL3
Funding
- University of Padova, Progetti di Ricerca di Ateneo [CPDA151400/15]
- HFSP [RGP0013/2014]
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Ras-selective lethal small molecule 3 (RSL3), a drug candidate prototype for cancer chemotherapy, triggers ferroptosis by inactivating the glutathione peroxidase glutathione peroxidase 4 (GPx4). Here, we report the purification of the protein indispensable for GPx4 inactivation by RSL3. Mass spectrometric analysis identified 14-3-3 isoforms as candidates, and recombinant human 14-3-3 epsilon confirms the identification. The function of 14-3-3 epsilon is redox-regulated. Moreover, overexpression or silencing of the gene coding for 14-3-3 epsilon consistently controls the inactivation of GPx4 by RSL3. The interaction of GPx4 with a redox-regulated adaptor protein operating in cell signaling further contributes to frame it within redox-regulated pathways of cell survival and death and opens new therapeutic perspectives.
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