Journal
BIOMACROMOLECULES
Volume 17, Issue 6, Pages 2168-2177Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.6b00398
Keywords
-
Funding
- Zhejiang Provincial Natural Science Foundation of China [LQ15E030003, Y14H150023, Y14H090062, LY13H030008, LY14H150008]
- Wenzhou Science & Technology Bureau of China [Y20140727]
- Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [YKFJ001]
- National Natural Science Funding of China [81372112, 81372064, 81302775, 81472165, 81571923]
- State Key Basic Research Development Program [2012CB518105]
- Zhejiang Provincial Project of Key Group [2010R50042]
- NSF [CBET-0952624, CBET-1510099, DMR- 1607475]
- Alzheimer Association [2015-NIRG-341372]
- National Natural Science Foundation of China [NSFC- 21528601]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0952624] Funding Source: National Science Foundation
Ask authors/readers for more resources
Effective wound healing requires complicated, coordinated interactions and responses at protein, cellular, and tissue levels involving growth factor expression, cell proliferation, wound closure, granulation tissue formation, and vascularization. In this study, we develop a heparin-based coacervate consisting of poly(ethylene argininylaspartate digylceride) (PEAD) as a storage matrix, heparin as a bridge, and fibroblast growth factor-2 (FGF2) as a cargo (namely heparin-FGF2@PEAD) for wound healing. First, in vitro characterization demonstrates the loading efficiency and control release of FGF2 from the heparin-FGF2@PEAD coacervate. The following in vivo studies examine the wound healing efficiency of the heparin-FGF2@PEAD coacervate upon delivering FGF2 to full-thickness excisional skin wounds in vivo, in comparison with the other three control groups with saline, heparin@PEAD as vehicle, and free FGF2. Collective in vivo data show that controlled release of FGF2 to the wounds by the coacervate significantly accelerates the wound healing by promoting cell proliferation, stimulating the secretion of vascular endothelial growth factor (VEGF) for re-epithelization, collagen deposition, and granulation. tissue formation, and enhancing the expression of platelet endothelial cell adhesion molecule (CD31) and alpha-smooth muscle actin (a-SMA) for blood vessel maturation. In parallel, no obvious wound healing effect is found for the control, vehicle, and free FGF2 groups, indicating the important role of the coavervate in the wound healing process. This work designs a suitable delivery system that can protect and release FGF2 in a sustained and controlled manner, which provides a promising therapeutic potential for topical treatment of wounds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available