Journal
DEVELOPMENTAL CELL
Volume 51, Issue 4, Pages 476-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2019.10.018
Keywords
-
Categories
Funding
- Graduate School of Quantitative Biosciences Munich
- European Commission [FP7 GA ERC-2012-SyG_318987-ToPAG, ERC AdG TENDO -834394]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) through Germany's Excellence Strategy [EXC 2067/1-390729940, SFB1190/P22]
- Spanish Ministry of Economy and Competitiveness (Severo Ochoa'' program for Centres of Excellence in RD) [SEV-2015-0522, FIS2015-63550-R, FIS2017-89560-R, BFU2015-73288-JIN, RYC-2017-22227]
- Spanish Ministry of Economy and Competitiveness (Severo Ochoa'' program for Centres of Excellence in R&D (AEI/FEDER/UE))
- Fundacio Privada Cellex
- Generalitat de Catalunya through the CERCA program
- Swiss National Science Foundation [31003A_179517]
- Swiss National Centre for Competence in Research in Chemical Biology [51NF40-185898]
- Canton of Geneva
- MRC [MC_UU_00012/6]
- MRC [MC_UU_00012/6] Funding Source: UKRI
- Swiss National Science Foundation (SNF) [31003A_179517] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are particularly abundant in Saccharomyces cerevisiae, where approximately half of the PM surface is covered by cortical ER (cER). Several proteins, including Ist2, Scs2/22, and Tcb1/2/3 are implicated in cER formation, but the specific roles of these molecules are poorly understood. Here, we use cryo-electron tomography to show that ER-PM tethers are key determinants of cER morphology. Notably, Tcb proteins (tricalbins) form peaks of extreme curvature on the cER membrane facing the PM. Combined modeling and functional assays suggest that Tcb-mediated cER peaks facilitate the transport of lipids between the cER and the PM, which is necessary to maintain PM integrity under heat stress. ER peaks were also present at other MCS, implying that membrane curvature enforcement may be a widespread mechanism to regulate MCS function.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available