Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 182, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.06.054
Keywords
Graphene; Neural stem cells; Differentiation; Transcriptomic profiling
Funding
- Major State Basic Research Development Program of China (973 Program) [2015CB965000, 2017YFA0104303]
- National Natural Science Foundation of China [31571530, 81622013, 81470692, 31500852, 31871322]
- Chinese Academy of Science [XDA16000000]
- BoehringerIngelheimPharma GmbH
- YingdongHuo Education Foundation
- Fundamental Research Funds for the Central Universities [2242017K41042, 2242017K3DN23, 2242017K41041]
- State Key Laboratory of Genetic Engineering, Fudan University [SKLGE1809]
- Scientific Research Foundation of the Graduate School of Southeast University [YBJJ1739]
- Fundamental Research Funds for the Central Universities
- Postgraduate Research&Practice Innovation Program of Jiangsu Province [KYCX17_0050]
Ask authors/readers for more resources
Graphene exhibits excellent mechanical strength, electrical conductivity and good biocompatibility, which make it a suitable candidate as a neural interfacing material in regenerative medicine and tissue engineering. Graphene is reported to promote both of neural stem cells (NSCs) proliferation and differentiation. However, the transcriptomes of 2D graphene-regulated NSC differentiation have not yet been investigated. To identify candidate genes, on which graphene may affect, we used next-generation RNA sequencing to analyze the transcriptome of NSCs differentiated for 21 days on a graphene substrate. These NSCs displayed highly enriched and differentially expressed genes compared with traditional cell culture in vitro. Of these, we identified motor protein genes that might regulate NSC differentiation, including cytoplasmic dynein and axonemal dynein genes, Ccdc108, Drtah5, and Dnah11. Furthermore, we analyzed the cell signaling pathway genes that might regulate NSC differentiation, and we constructed a protein-protein interaction network for the genes that are differentially expressed in NSCs on graphene compared to commercial tissue culture polystyrene substrates. We have identified genes potentially regulating the differentiation and migration of NSCs on graphene substrates, and our findings provide mechanistic evidence for the biological activities of graphene, especially in view of graphene-stem cell interactions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available