Journal
CELLULAR SIGNALLING
Volume 62, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2019.05.020
Keywords
Immunofluorescence; Histochemistry; Automatic quantification; Cell count; Morphology
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Funding
- Light Microscopy Facility, a Core Facility of the CMCB Technology Platform at Technische Universitat Dresden
- CFCI (Core Facility Cellular Imaging) at the Carl Gustav Carus Faculty of Medicine
- DFG [GE 2845/1-1, TO 679/2-1, TO 679/3-1, HU 600/6-1, HU 600/8-1]
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Immunofluorescent staining is a widespread tool in basic science to understand organ morphology and (patho-) physiology. The analysis of imaging data is often performed manually, limiting throughput and introducing human bias. Quantitative analysis is particularly challenging for organs with complex structure such as the kidney. In this study we present an approach for automatic quantification of fluorescent markers and histochemical stainings in whole organ sections using open source software. We validate our novel method in multiple typical challenges of basic kidney research and demonstrate its general relevance and applicability to other complex solid organs for a variety of different markers and stainings. Our newly developed software tool AQUISTO, applied as a standard in primary data analysis, facilitates efficient large scale evaluation of cellular populations in various types of histological samples. Thereby it contributes to the characterization and understanding of (patho-) physiological processes.
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