Journal
CARBOHYDRATE POLYMERS
Volume 230, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115635
Keywords
Chitosan; Arginine; Guanidyl; Modification; Water solubility; Antibacterial properties
Categories
Funding
- National Key R&D Program of China [2018YFD0901106]
Ask authors/readers for more resources
In this study, 6-deoxy-6-arginine modified chitosan (DAC), was synthesized and characterized by Fourier Transform Infrared Spectroscopy (FTIR), H-1 and C-13 nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and elemental analysis. The arginine was grafted onto C6 groups of chitosan. Antibacterial activity of DAC against gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated at concentration between 0.02 mg/mL and 10 mg/mL. Cell viability assessment was estimated in vitro with Caco-2 and L929 cells. Water solubility of DAC at different pH was also evaluated. The results showed that the minimum inhibitory concentration (MICs) of DAC against S. aureus and E. coli. were 0.078 mg/mL and 0.312 mg/mL, respectively. The minimum bactericidal concentration (MBC) against S. aureus and E. coli was 0.625 mg/mL. The cytotoxicity of chitosan and DAC was not significantly different. It demonstrated that DAC might be a potential safe antibacterial agent.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available