Journal
CARBOHYDRATE POLYMERS
Volume 224, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115185
Keywords
High amylose starch; Starch structure; Branching enzyme; Wheat; Chain-length distribution
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Funding
- Priority Academic Program of Jiangsu Higher Education Institutions
- Australian Research Council [LP160100310]
- Natural Science Foundation of China [C1304013151101138]
- 2017 Jiangsu Innovation and Entrepreneurship talents program
- University of Queensland Research Training Scholarship
- Australian Research Council [LP160100310] Funding Source: Australian Research Council
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Starch branching enzymes (SBEs) play a major role in determining starch molecular structure in cereal endosperm. This study investigates how SBEIIs contribute to the chain-length distributions (CLDs) of both amylopectin and amylose, obtained by enzymatic debranching of native starch. Wheat starches with low (37%) to high (93%) amylose content were obtained through altering SBEII in planta. Multiple components were detected in both amylose and amylopectin CLDs. Model fitting of these CLDs reveals a quantitative association between the enzyme activities in amylopectin and amylose. SBEIIa modifies shorter branches (degree of polymerization DP less than or similar to 12) in amylopectin and longer amylose chains with a CLD peak at 3000 DP. SBEIIb acts on longer branches (DP less than or similar to 32) in amylopectin, while its effect on amylose fine structure is not significant. Using both the amylose and amylopectin models to analyze the CLD reveals connections between amylose and amylopectin in wheat starch biosynthesis.
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