Journal
CARBOHYDRATE POLYMERS
Volume 221, Issue -, Pages 120-126Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.05.086
Keywords
Marine bacterium; Vadicella arenosi; O-polysaccharide; Sulfated fucan; Antiproliferative activity; Cancer preventive properties
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Funding
- Program of fundamental research of the Far Eastern Branch of Russian academy of Sciences Far East 2018-2020 [18-4-036]
- RFBR [18-004-00618]
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Sulfated fucose-containing glycopolymers are currently of great interest because of their wide spectrum of bioactivity, including anti-tumor properties. In this study, the structure of O-polysaccharide (OPS) of the marine bacterium Vadicella arenosi KMM 9024(T), its effect on the proliferation of human breast cancer MCF-7 cells and cancer preventive properties were investigated. Two OPS fractions with different molecular weights were isolated and purified from the lipopolysaccharide by mild acid hydrolysis followed by anion-exchange chromatography. The OPS was found to consist of alpha-(1 -> 3)-linked 2-O-sulfate-D-fucopyranosyl residues, whose structure was deduced by sugar analysis along with 2D NMR spectroscopy. The biological assay indicated that polysaccharide significantly reduced the proliferation and inhibited colony formation of MCF-7 cells in a dose-dependent manner. Besides, the experiment indicated the inhibitory role of polysaccharide on EGF-induced neoplastic cell transformation in mouse epidermal cells. The investigated polysaccharide is the first sulfated fucan isolated from the bacteria.
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