Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 117, Issue 2, Pages 593-598Publisher
WILEY
DOI: 10.1002/bit.27199
Keywords
CHO; CRISPRa; glycosylation; Mgat3; St6gal1
Categories
Funding
- Novo Nordisk Foundation [NNF10CC1016517, NNF16OC0021638]
- National Institute of General Medical Sciences [R35 GM119850]
Ask authors/readers for more resources
Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced. This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available