Journal
BIOSENSORS & BIOELECTRONICS
Volume 144, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111697
Keywords
Surface plasmon resonance (SPR); Carcinoembryonic antigen (CEA); Immunoassay; 2D materials; MXene
Categories
Funding
- National Postdoctoral Program for Innovative Talents [BX20180117]
- National Key Research and Development Program of China [2016YFA0200400]
- National Natural Science Foundation of China [51571100, 51872116, 51702116, 51602305, 31771093]
- Program for JLU Science and Technology Innovative Research Team (JLUSTIRT) [2017TD-09, 2017TD-27, 2019TD-36]
- Project of International Collaboration of Jilin Province [201180414085GH]
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Surface plasmon resonance (SPR) has become a leading technique for in situ bioaffinity assay of diverse targets without need of fluorescent or enzymatic labeling. Nanomaterials-enhanced SPR sensors have developed rapidly and widened the application scope of SPR sensing technology. In this report we describe an ultrasensitive SPR biosensor for detecting carcinoembryonic antigen (CEA). Our SPR biosensor utilizes a Ti3C2-MXene-based sensing platform and multi-walled carbon nanotube (MWCNTs)-polydopamine (PDA)-Ag nanoparticle (AgNPs) signal enhancer. Ti3C2-MXene, a new class of two-dimensional (2D) transition metal carbides, offers a large hydrophilic-biocompatible surface ideal for SPR biosensing. Ti3C2-MXene/AuNPs composites after synthesis are then decorated with staphylococcal protein A (SPA) to orient and immobilize monoclonal anti-CEA antibody (Ab(1)) through its Fc region. By introducing MWCNTs-PDA-AgNPs-polyclonal anti-CEA antibody (MWPAg-Ab(2)) conjugate combined with a sandwich format, the present method provides a dynamic range for CEA determination of 2 x 10(-16) to 2 x 10(-8) M and a detection limit of 0.07 fM. This biosensing approach demonstrates good reproducibility and high specificity for CEA in real serum samples providing a promising method to evaluate CEA in human serum for early diagnosis and monitoring of cancer.
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