Enhancement of bio-hydrogen yield and pH stability in photo fermentation process using dark fermentation effluent as succedaneum

The photo fermentation hydrogen yield from dark fermentation effluents (DFEs) can be promoted by adding corn straw enzymatic hydrolysate adjusts the nutritional composition of DFEs. As compared with the control group (without enzymatic hydrolysate addition), the effect of adding enzymatic hydrolysate make H-2 yield increase from 312.54 to 1287.06 mL H-2/g TOC, and maximum hydrogen production rate increase 2.14 to 10.23 mL/h. On the other hand, buffer reagents remained in DFEs make which can replace part sodium citrate buffer to maintain pH stability in synchronized saccharification and photosynthetic fermentation process with corn straw as substrate, the best result was observed at the ration of 1:2 (33 mL DFEs, 67 mL sodium citrate buffer) with the hydrogen yield of 436.30 +/- 10 mL, and which can cut down the GHG in the life cycle of hydrogen production.