Studies of advanced lignin valorization based on various types of lignolytic enzymes and microbes

Lignin is a robust material that is considered useless because it has an inhibitory effect on microbes and acts as a physical barrier for cellulose degradation. Therefore, it has been removed from cellulosic biomass to produce high-value materials. However, lignin monomers can be converted to value-added chemicals such as biodegradable plastics and food additives by appropriately engineered microbes. Lignin degradation through peroxidase, laccase and other proteins with auxiliary activity is the first step in lignin valorization. Metabolic engineering of microorganisms for increased tolerance and production yield is the second step for lignin valorization. Here, this review offers a summary of current biotechnologies using various enzymatic activities, synergistic enzyme mixtures and metabolic engineering for lignin valorization in biorefinery.