Journal
BIORESOURCE TECHNOLOGY
Volume 289, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.121694
Keywords
Astaxanthin; Pulsed electric field (PEF); Microalgae; Incubation; Extraction
Funding
- COST Action [ES1408]
- Swedish Energy Agency [2018-017772, 48007-1]
- Vinnova [2017-03301]
- NordForsk NCoE programme NordAqua [82845]
- Umea University
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The Nordic microalgal strain Haematococcus pluvialis was exposed to various stress conditions to induce astaxanthin accumulation. Highest carotenoid content (19.1 mg.g(dw)(-1)) was achieved in nitrogen-free culture medium at a high light intensity. The efficiency of Pulsed Electric Field (PEF) pre-treatment of stressed fresh biomass of H. pluvialis followed by incubation in the growth medium was compared to classical disruption methods (bead-beating, freezing-thawing, thermal treatment or ultrasound) for the subsequent extraction of astaxanthin in ethanol. N-starved cells treated with PEF followed by aqueous incubation for 6 h resulted in extraction of 96% (18.3 mg(car).g(dw)(-1)) of the total carotenoid content compared to 80% (15.3 mg(car).g(dw)(-1)) using other physical methods. The proportion of free forms of astaxanthin was higher in PEF-treated samples compared to mechanical disruption, suggesting PEF triggering an esterase activity. PEF pre-treatment of the cells followed by incubation in growth medium improved astaxanthin extraction in the eco-friendly solvent ethanol.
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