Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 43, Issue 2, Pages 293-301Publisher
SPRINGER
DOI: 10.1007/s00449-019-02226-2
Keywords
Monocrotophos; Carbon nanotubes; Horseradish peroxidase; Acetylcholinesterase; Biosensor
Funding
- China Postdoctoral Science Foundation [2014M550271] Funding Source: Medline
- National Natural Science Foundation of China [21406093] Funding Source: Medline
- Natural Science Foundation of Jiangsu Province [BK20140529] Funding Source: Medline
- the Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University [SKLF-KF-201919] Funding Source: Medline
- Key University Science Research Project of Jiangsu Province [14KJB530001] Funding Source: Medline
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Long-term and excessive use of monocrotophos (MPs) pesticide leads to an accumulation of MPs residues in agricultural products. Electrochemical biosensor technology was developed as a simple and efficient method for detecting MPs. However, commercial acetylcholinesterase (AChE) sensors are not applied in practical MPs detection due to poor stability and reliability. In this study, the advantages of functionalized carbon nanotubes (Cl/MWCNTs) and a bi-enzyme system (horseradish peroxidase (HRP)/AChE) were combined, a novel bi-enzyme electrode (Cl/MWCNTs/HRP/AChE/GCE) was constructed. Under optimal conditions, the bi-enzyme sensor had a wide detection range of 1.0 x 10(-11) to 1.0 x 10(-7) mol/L and low detection limit of 4.5 x 10(-12) mol/L. The proposed AChE biosensor exhibited excellent stability and sensitivity for MPs determination and presented a promising tool for monitoring food safety.
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