Journal
BIOPHYSICAL CHEMISTRY
Volume 254, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bpc.2019.106262
Keywords
Surface plasmon resonance; Grating; Nanostructure; Live cells
Funding
- Fondazione Istituto di Ricerca Pediatrica [MyFirstIRP 19/06IRP]
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The application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.
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