Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 519, Issue 1, Pages 29-34Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2019.08.090
Keywords
GDF15; NAG-1; Nuclear localization signal; Peptide transfection; Mitochondrial membrane potential; High-content screening
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Funding
- Research Institute for Veterinary Science
- BK21 PLUS Program for Creative Veterinary Science Research Center, Seoul National University
- National Research Foundation of Korea (NRF) - Korea government [NRF-2018R1A2B2002923]
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Non-steroidal anti-inflammatory drug activated gene-1 (NAG-1), also known as growth differentiation factor 15 (GDF15), is a TGF-beta (transforming growth factor beta) superfamily protein with a distinctive secretion pathway. NAG-1 is associated with multiple diseases including cancer, wherein it plays a role in both pro- and anti-cancer activities. We previously reported that NAG-1 is translocated to different subcellular compartments and its activity depends on its localization. In this paper, we report that the transfection of a novel peptide corresponding to the nuclear localization signal (NLS) of NAG-1 blocks its translocation to the nucleus. Further, accumulation of NAG-1 in the cytoplasm decreased mitochondrial membrane potential, thus implying apoptosis induction as a consequence. Overall, our results indicate that the novel peptide derived from NAG-1 NLS sequence is a promising tool for enhancing the anti-tumorigenic activity of NAG-1. (C) 2019 Elsevier Inc. All rights reserved.
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