Journal
BIOELECTROMAGNETICS
Volume 37, Issue 4, Pages 212-222Publisher
WILEY-BLACKWELL
DOI: 10.1002/bem.21968
Keywords
geomagnetic field; ATP; ROS; energy production; mitochondrial membrane potential
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Funding
- National Nature Science Foundation of China [31200628]
- Chinese Academy of Sciences [YZ201148]
- BIC, Chinese Academy of Sciences [GJHZ201302]
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Hypomagnetic field (HMF), one of the key environmental risk factors for astronauts traveling in outer space, has previously been shown to repress locomotion of mammalians. However, underlying mechanisms of how HMF affects the motor system remains poorly understood. In this study, we created an HMF (<3T) by eliminating geomagnetic field (GMF, approximate to 50T) and exposed primary mouse skeletal muscle cells to this low magnetic field condition for a period of three days. HMF-exposed cells showed a decline in cell viability relative to GMF control, even though cells appeared normal in terms of morphology and survival rate. After a 3-day HMF-exposure, glucose consumption of skeletal muscle cells was significantly lower than GMF control, accompanied by less adenosine triphosphate (ATP) and adenosine diphosphate (ADP) content and higher ADP/ATP ratio. In agreement with these findings, mitochondrial membrane potential of HMF-exposed cells was also lower, whereas levels of cellular Reactive Oxygen Species were higher. Moreover, viability and membrane potential of isolated mitochondria were reduced after 1h HMF-exposure in vitro. Our results indicate that mitochondria can directly respond to HMF at functional level, and suggest that HMF-induced decline in cell functionality results from a reduction in energy production and mitochondrial activity. Bioelectromagnetics. 37:212-222, 2016. (c) 2016 Wiley Periodicals, Inc.
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