Journal
ANTICANCER RESEARCH
Volume 39, Issue 11, Pages 6073-6086Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.13815
Keywords
Adenovirus; cancer stem cells; DNA internalization; TAMRA; U87 cell line
Categories
Funding
- State Project of the Institute of Cytology and Genetics [0324-2019-0042]
- Novosibirsk State University [6.5546.2017]
- Russian Foundation for Basic Research [18-34-20016]
- Novosibirsk State University
Ask authors/readers for more resources
Background/Aim: Oncolytic adenoviruses are promising therapeutic agents against both the bulk of tumor cells and cancer stem cells. The present study intended to test the oncolytic capability of adenovirus serotype 6 (Ad6), which has a lower seroprevalence and hepatotoxicity relatively to adenovirus 5 (Ad5), against the glioblastoma and its cancer stem cells. Materials and Methods: Oncolytic efficacy of Ad6 was compared to widespread Ad5 both in vitro and in vivo, using the U87 and U251 human glioblastoma cell lines and subcutaneously transplanted U87 cells in SCID mice, respectively. Results: Ad6 had a dose-dependent cytotoxicity toward glioblastoma cells in vitro and its intratumoral injections lead to a significant (p< 0.05) decrease in volume of U87 xenografts, similarly to Ad5. Based on the innate capability of glioblastoma cancer stem cells to internalize a fluorescent-labeled double-stranded DNA probe, the spatial localization of these cells was estimated and it was shown that the number of cancer stem cells tended to decrease under adenovirus therapy as compared to the control group. Conclusion: Ad6 was shown to be a promising agent for treating glioblastomas.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available