Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 43, Pages 15448-15454Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201906438
Keywords
bacteria phenotype; droplet microfluidics; stochastic DNA walkers; super-multiplex detection
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Funding
- National Science Foundation of China [21722502]
- Shanghai Rising-Star Program [19QA1403000]
- Shanghai Science and Technology Committee (STCSM) [18490740500]
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Pathogen detection is growing in importance in the global health arena because of the high morbidity and mortality associated with bacterial blood stream infections. In this work, we present stochastic DNA walkers in droplets (SDwalker-Drop), a one-step, rapid, and super-multiplex method for ultrahigh-throughput bacterial detection. The SDwalkers, by exploiting cascade signal amplification, endow our analytical platform with fast analysis times and single-cell analysis ability. The autonomous and multiple-step walking behavior of the SDwalkers provides a super-multiplex droplet-encoding strategy by embedding intensity coded barcodes into a sequence of color-multiplexed barcodes. We realized a theoretical coding capacity of 8(3)-1=511 and achieved 20 distinct patterns for bacterial phenotype detection and identification. Moreover, our SDwalker-Drop platform could be readily integrated with a flow cytometer to afford a general approach for super-multiplexed, high-throughput biological assays and screening.
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